omx-3d superresolution microscope v3 Search Results


96
Cytiva Europe deltavision omx 3d sim sr
Deltavision Omx 3d Sim Sr, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Applied Precision Inc omx 3d sim microscope
The ring complex has internal domains that weave through Meiosis I bivalents. (A) Meiosis I bivalents (Top) and Meiosis II chromosomes (Bottom); shown are DNA (blue), SUMO (green), and AIR-2 (red). Zooms highlight individual RCs; MI bivalents have an internal substructure (arrow; 57/57) while MII chromosomes do not (0/54 have internal RC components). <t>(B)</t> <t>3D-SIM</t> superresolution image of an end-on Meiosis I bivalent; shown are DNA (blue), SUMO (green), and AIR-2 (red). Arrows highlight subloops within the RC. (C) 3D-SIM superresolution image of a Metaphase I-arrested monopolar spindle. This condition was generated by performing klp-18;klp-7 MCAK (RNAi) in emb-27(g48)ts worms and shifting them to the restrictive temperature. AIR-2 (red) stretches toward the plus ends of the microtubules, which reveals subloops (arrows) associated with the bivalent; subloops are apparent in the single-slice zoom (schematic shows RC organization). (D) Model for RC organization in Meiosis I. Homologous chromosomes are shown in light/dark blue and cohesin linking sisters is shown in purple. After homologs pair and a single off-centered crossover occurs, the cruciform structure condenses such that the short arms are indistinguishable from the long arms. The RC (red) forms subloops around the short arms of the bivalents as well as encircling the outside of the bivalent. Scale bars = 5 µm (full images), 1 µm (zooms).
Omx 3d Sim Microscope, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The ring complex has internal domains that weave through Meiosis I bivalents. (A) Meiosis I bivalents (Top) and Meiosis II chromosomes (Bottom); shown are DNA (blue), SUMO (green), and AIR-2 (red). Zooms highlight individual RCs; MI bivalents have an internal substructure (arrow; 57/57) while MII chromosomes do not (0/54 have internal RC components). (B) 3D-SIM superresolution image of an end-on Meiosis I bivalent; shown are DNA (blue), SUMO (green), and AIR-2 (red). Arrows highlight subloops within the RC. (C) 3D-SIM superresolution image of a Metaphase I-arrested monopolar spindle. This condition was generated by performing klp-18;klp-7 MCAK (RNAi) in emb-27(g48)ts worms and shifting them to the restrictive temperature. AIR-2 (red) stretches toward the plus ends of the microtubules, which reveals subloops (arrows) associated with the bivalent; subloops are apparent in the single-slice zoom (schematic shows RC organization). (D) Model for RC organization in Meiosis I. Homologous chromosomes are shown in light/dark blue and cohesin linking sisters is shown in purple. After homologs pair and a single off-centered crossover occurs, the cruciform structure condenses such that the short arms are indistinguishable from the long arms. The RC (red) forms subloops around the short arms of the bivalents as well as encircling the outside of the bivalent. Scale bars = 5 µm (full images), 1 µm (zooms).

Journal: Molecular Biology of the Cell

Article Title: Newfound features of meiotic chromosome organization that promote efficient congression and segregation in Caenorhabditis elegans oocytes

doi: 10.1091/mbc.E22-07-0297

Figure Lengend Snippet: The ring complex has internal domains that weave through Meiosis I bivalents. (A) Meiosis I bivalents (Top) and Meiosis II chromosomes (Bottom); shown are DNA (blue), SUMO (green), and AIR-2 (red). Zooms highlight individual RCs; MI bivalents have an internal substructure (arrow; 57/57) while MII chromosomes do not (0/54 have internal RC components). (B) 3D-SIM superresolution image of an end-on Meiosis I bivalent; shown are DNA (blue), SUMO (green), and AIR-2 (red). Arrows highlight subloops within the RC. (C) 3D-SIM superresolution image of a Metaphase I-arrested monopolar spindle. This condition was generated by performing klp-18;klp-7 MCAK (RNAi) in emb-27(g48)ts worms and shifting them to the restrictive temperature. AIR-2 (red) stretches toward the plus ends of the microtubules, which reveals subloops (arrows) associated with the bivalent; subloops are apparent in the single-slice zoom (schematic shows RC organization). (D) Model for RC organization in Meiosis I. Homologous chromosomes are shown in light/dark blue and cohesin linking sisters is shown in purple. After homologs pair and a single off-centered crossover occurs, the cruciform structure condenses such that the short arms are indistinguishable from the long arms. The RC (red) forms subloops around the short arms of the bivalents as well as encircling the outside of the bivalent. Scale bars = 5 µm (full images), 1 µm (zooms).

Article Snippet: Images in Figure 1, B and C (denoted as superresolution) were acquired on an OMX 3D-SIM microscope (Applied Precision) with an Olympus 100× UPlanSApo objective (NA = 1.4) in the Center for Advanced Microscopy at Northwestern University.

Techniques: Generated